Quantification of splice variants using real-time PCR
نویسندگان
چکیده
منابع مشابه
Quantification of splice variants using real-time PCR
A reliable and robust method for measuring the expression of alternatively spliced transcripts is an important step in investigating the significance of each variant. So far, accurate quantification of splice variants has been laborious and difficult due to the intrinsic limitations of conventional methods. The many advantages of real-time PCR have made this technique attractive to study its ap...
متن کاملReal-time reverse transcription-PCR quantification of vascular endothelial growth factor splice variants.
Vascular Endothelial Growth Factor Splice Variants, Eleni Zygalaki, Aliki Stathopoulou, Christos Kroupis, Loukas Kaklamanis, Zenon Kyriakides, Dimitrios Kremastinos, and Evi S. Lianidou ( Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Athens, Greece; 2 Onassis Cardiac Surgery Center, Athens Greece; 3 Red Cross Hospital, Athens, Greece; * address correspondenc...
متن کاملQuantification and Optimization of Candida albicans DNA in Blood Samples Using Real- Time PCR
Background: Candida albicans (C. albicans) is a major cause of candidaemia in people with impaired immunity. Blood culture is a “gold standard” for candidaemia detection but is time-consuming and relatively insensitive. We established a real-time PCR assay for C. albicans detection in blood by LightCycler PCR and melting curve analysis. Methods: Five milliliter blood samples from...
متن کاملDNA quantification using EvaGreen and a real-time PCR instrument.
DNA quantiWcation is an important, frequently used technique, and inaccuracies can result in failures with ligation, restriction, polymerase chain reaction (PCR),1 ampliWed fragment length polymorphism (AFLP), Southern blotting, and other techniques. DNA is most commonly quantiWed using absorbance at 260 nm, but because of the existence of many impurities, this can be an imprecise measurement a...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2001
ISSN: 1362-4962
DOI: 10.1093/nar/29.13.e68